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. 2018 Nov 2;8:16273. doi: 10.1038/s41598-018-34591-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of BrYV amplicon-transformed Arabidopsis. (a) Southern blot analysis indicating that the cDNA of BrYV was inserted into the Arabidopsis genome. The genomic DNA of transgenic Arabidopsis plants was digested with either EcoR I or Hind III. (b) Northern blot results showing the constitutive expression of BrYV-encoded genomic RNA (gRNA) and subgenomic RNAs (sgRNAs). (c) Western blot analysis demonstrating the expression of the BrYV coat protein in lines 111 and 412.