Figure 6.

Ectopic expression of MP^BrYV changes the phenotype of transgenic Arabidopsis lines. (a) Molecular analysis of transgenic lines (#1, #2, and #3) that consistently expressing the MP^BrYV. For PCR detection the positive control was the pCaBrC plasmid. For RT-PCR detection, a sample of BrYV-infected Nicotiana benthamiana was used for positive control. For the western blot analysis, the positive control was a sample transiently expressing the FLAG-tagged P0 protein. (b) RT-qPCR analysis of DFR expression levels in #1, #2, and #3. Mean ± SD of n = 3 independent experiments. **P < 0.01. (c) Morphological characteristics of MP^BrYV-transformed Arabidopsis lines. Upper: 8-week-old leaves of Col-0, #1, #2, and #3; bottom: 8-week-old plants of Col-0 and the three MP^BrYV-transformed Arabidopsis lines.