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. 2018 Nov 2;8:16244. doi: 10.1038/s41598-018-34292-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Box plot depicts the overall expression level across samples after normalization. (blue bar: normal samples, red bar: GDM samples). (B) Heatmap shows the differential expressed genes’ expression profiles across samples under the standard of p < 0.05. Red in the scale bar represents high expression level, and green means relatively low expression level. c. Heatmap shows the 182 differential CpG methylation sites filtered by a more stringent criteria of p < 0.05 and |delta beta| > 0.2. Red in the scale bar represents sites with relatively higher methylation level and and green is the opposite. d. Cluster analysis of GO terms.