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. 2018 Nov 2;8:16289. doi: 10.1038/s41598-018-34511-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identified drugs enhance osteogenesis in hMSCs from aged donor and at older passage number. (A) and (B) Influence of identified drugs on osteogenic differentiation was analyzed using ALP activity assay in cells from an older donor (age 46 years) and in cells at a late passage number (p7), respectively. Statistical analysis was performed using the Dunnett Multiple Comparisons test, which compares all columns versus a control column (no treatment). The symbol ** represent a significant change in ALP activity with respect to no treatment condition to the level of p < 0.01 and p < 0.05, respectively.