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. 2018 Oct 24;14:8–16. doi: 10.1016/j.ctro.2018.10.004

Fig. 3.

Fig. 3

FGF1/CPP-C inhibits metastatic capabilities of pancreatic carcinoma cell lines. (A) The invasiveness of MIAPaCa-2 and PANC-1 cells was examined by invasion assays 24 h after the incubation in Matrigel-coated transwells with 100 ng/ml of FGF1 or FGF1/CPP-C in the presence of 5 μg/ml heparin. Invading cells on the transwell membrane are shown. (B) The number of invading cells was assessed using the public domain ImageJ program (NIH, Bethesda, MD) and the ratio of invading cells was obtained by dividing them by the total number of seeded cells. All values are means ± SD (n = 4). **P < 0.01; ***P < 0.001. (C) The migration of MIAPaCA-2 and PANC-1 cells was tracked by the wound healing assay using IncuCyte (Essen Bioscience, Ann Arbor, MI, USA) during the culture in a 96-well-plate with 100 ng/ml of FGF1 or FGF1/CPP-C. The migration rate of cells was assessed after 24- and 48-h cultures. All values are means ± SD (n = 3). *P < 0.05.