Fig. 4.

Methanol-induced ethylene synthesis in cultured cells. Methanol-induced synthesis of ethylene in tobacco cells after a 4 h treatment and its inhibition by pre-treatment with 200 µm α-aminoisobutyric acid (AIB), an inhibitor of ACC oxidase, or 200 µm aminooxyacetic acid (AOA), an inhibitor of ACC synthase. Pre-treatment with a Ca²⁺ channel inhibitor (500 µm La³⁺) or a calcium chelator (1 mm BAPTA) also reduced the methanol-induced ethylene production after 4 h. Data are expressed as picomoles of ethylene produced per gram of fresh weight, and are representative of at least four independent experiments, and error bars correspond to the standard errors. *Significantly different from the control level (addition of 5 µL H₂O).