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. 2018 Oct 10;10(10):408. doi: 10.3390/toxins10100408

Figure 6.

Figure 6

Effect of JZTx-14 on NaChBac mutants. (A) Sequence alignment of NaChBac with several bacterial NaVs determined the S3–4 extracellular loops. The mutation sites are labeled by red arrows. (B–G) Representative traces showing the inhibitory effect of 1 µM JZTx-14 on wild-type NaChBac and NaChBac mutants. (H) Dose–response curves for JZTx-14 inhibiting the currents of NaChBac mutants. The IC50 values were 320.0 ± 38.0 nM, 832.6 ± 42.1 nM, 653.3 ± 92.1 nM, 809.8 ± 87.5 nM, 3472.3 ± 195.7 nM, and 1367.3 ± 129.3 nM for wild-type NaChBac, F103A, G105A, Q107A, F108A, and V109A, respectively (n = 5–7). (I) The bars show the fold changes of the IC50 values of mutant channels when compared with wild-type NaChBac. (J,K) Representative traces showing the inhibitory effect of 300 nM JZTx-27 on wild-type NaChBac and F108A mutant channel. (L) Dose–response curves for JZTx-27 inhibiting the currents of the wild-type NaChBac and F108A mutant channels (n = 6–7).