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. 2018 Oct 13;10(10):414. doi: 10.3390/toxins10100414

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Role of LtxA in OMV association and toxicity. (A) Association of JP2 (⬛) and AA1704 (⚪) OMVs with THP-1 cells. ANOVA indicates no significant difference in the two data sets. (B) Activity of OMV-associated LtxA. THP-1 cells were incubated with PBS, JP2 OMVs, AA1704 OMVs, or LtxA for 16 h and viability was measured using an MTT assay. Data represent mean ± standard deviation (SD) with n = 5. NS^*, not significant; ***, p < 0.001 relative to phosphate buffered saline (PBS) control. NS^†, not significant; †††, p < 0.001 relative to JP2 OMVs.