Skip to main content
. 2018 Oct 11;10(10):410. doi: 10.3390/toxins10100410

Figure 2.

Figure 2

Cytosolic and mitochondrial ROS generating mechanisms start the persistent ROS production induced by transient exposure to 20 mM urea in endothelial cells. (A) In the indicated groups, HAEC were infected with MnSOD, or control adenoviral vectors before transient exposure to 20 mM glucose, or incubated for 48 h in 20 mM urea in the presence of the NADPH oxidase inhibitor GKT137831. ROS levels were measured by CM-H2DCFDA. (B) MnSOD protein level detected in HAEC 96 h after cells were infected with MnSOD adenovirus (MOI 500) compared to MnSOD level detected in cells infected with empty vector. Data are the mean ± S.E. from five independent experiments. * p < 0.05 compared to cells treated in the same conditions but not exposed to urea, $ p < 0.05 cells transfected with the empty vector compared to cells over expressing MnSOD, § p < 0.05 cells not transfected or not treated with the inhibitor compared to cells treated with GKT13781.