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. 2018 Oct 11;10(10):410. doi: 10.3390/toxins10100410

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Transient exposure to 20 mM urea induces a persistent reduction of mtDNA copy number in endothelial cells that is reversed by MnTBAP treatment. (A) HAEC were exposed to 20 mM Urea (HU) for 48 h , and to 20 mM urea followed by two days of subsequent incubation in media without urea (NU) . In the indicated groups, cells were infected with MnSOD before transient exposure to urea (A), or were treated with MnTBAP during the two days of subsequent incubation in media alone (B). mtDNA copy number was measured by a quantitative Real-time polymerase chain reaction (PCR)-based method. Data are the mean ± S.E. from seven independent experiments. * p < 0.05 compared to cells treated in the same conditions but not exposed to urea, $ p < 0.05 cells transfected with the empty vector compared to cells over expressing MnSOD, # p < 0.05 cells not treated with the inhibitor compared to cells treated with MnTBAP.

Inline graphic — Transient exposure to 20 mM urea induces a persistent reduction of mtDNA copy number in endothelial cells that is reversed by MnTBAP treatment. (A) HAEC were exposed to 20 mM Urea (HU) for 48 h , and to 20 mM urea followed by two days of subsequent incubation in media without urea (NU) . In the indicated groups, cells were infected with MnSOD before transient exposure to urea (A), or were treated with MnTBAP during the two days of subsequent incubation in media alone (B). mtDNA copy number was measured by a quantitative Real-time polymerase chain reaction (PCR)-based method. Data are the mean ± S.E. from seven independent experiments. * p < 0.05 compared to cells treated in the same conditions but not exposed to urea, $ p < 0.05 cells transfected with the empty vector compared to cells over expressing MnSOD, # p < 0.05 cells not treated with the inhibitor compared to cells treated with MnTBAP.