Table 1.

ISSR primers used for detecting somaclonal variation in V. planifolia.

Primer	Sequence (5′–3′)	°Tm (°C) 2	No. of Bands	Range (bp) 3	Polymorphism (%)
UBC 809	AGAGAGAGAGAGAGAGG	45	10	300–2000	30
T 06	AGAGAGAGAGAGAGAGGT	50	9	300–1550	66.66
UBC 840	GAGAGAGAGAGAGAGAYT 1	50	8	200–1400	25
UBC 836	AGAGAGAGAGAGAGAGYA 1	50	7	200–1550	42.86
UBC 812	GAGAGAGAGAGAGAGAA	50	7	200–1400	28.57
UBC 825	ACACACACACACACACT	51	6	500–3000	83.33
UBC 808	AGAGAGAGAGAGAGAGC	52	10	200–750	60
T 05	CGTTGTGTGTGTGTGTGT	54	8	300–2000	25
C 07	GAGAGAGAGAGAGAGAC	56	7	300–1400	71.43

¹ Y″: C or T residues. ² °Tm: annealing temperature. ³ bp: base pair.