Table 3.
Cytotoxic and Genotoxic effect of AgNPs on V. planifolia growth in vitro for six weeks.
| AgNPs (mg L−1) 1 | Cells in Division | Mitotic Index (%) | Aberration (%) 2 | Total Aberration (%) | |||
|---|---|---|---|---|---|---|---|
| CB | CF | BC | MN | ||||
| 0 | 2582 ± 92 * | 88.21 ± 0.48 * | 0.03 | 0 | 0 | 0 | 0.03 ± 0.00 * |
| 25 (1.5) | 2348 ± 75 * | 83.18 ± 1.16 * | 0.10 | 0.08 | 0 | 0 | 0.18 ± 0.06 * |
| 50 (3.0) | 2338 ± 87 * | 82.15 ± 2.40 * | 0.30 | 0.50 | 0.17 | 0 | 0.97 ± 0.03 ** |
| 100 (6.0) | 1786 ± 99 ** | 60.35 ± 0.90 ** | 1.5 | 1.0 | 0.16 | 1.5 | 4.16 ± 0.17 *** |
| 200 (12.0) | 1018 ± 72 *** | 33.53 ± 1.91 *** | 1.5 | 1.4 | 1.0 | 3.0 | 6.90 ± 0.22 **** |
1 Values in brackets correspond to the metallic silver content in AgNPs formulation. 2 The total number of cells counted was 3000. CB: Cells with bridges; CF: Chromosomal fragments; BC: Binucleated cells; MN: Micronuclei. Average values ± standard error within a column followed by the same number of asterisks are not significantly different according to Tukey’s test at p ≤ 0.05.