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. 2018 Oct 13;10(10):413. doi: 10.3390/toxins10100413

Figure 2.

Figure 2

(A) RP-HPLC of skin secretion of Hylarana guentheri at a wavelength of 214 nm. The abscissa was ‘retention time (min)’, and the ordinate ‘AU’ was ‘absorbance’. The arrow indicated the retention time of Brevinin-1GHa. (B) Predicted b-ion and y-ion MS/MS fragment ion series (singly- and doubly-charged) of Brevinin-1GHa. The actual ions detected in MS/MS spectra are colored red and blue. #1, the sequence from N-terminal to C-terminal; b(1+) and b(2+), singly- and doubly-charged b-ions; Seq.,the sequence of amino acid residues. y(1+) and y(2+), singly- and doubly-charged y-ions; #2, the sequence from C-terminal to N-terminal.