Table 4.
Structural parameters and minimum inhibitory concentrations (MICs) of Brevinin-1GHa and relevant AMPs homologs against specified microorganisms
| Peptide Name | GRAVY | pI | Net Charge | <H> | <𝛍H> | Antimicrobial Activity (MIC 𝛍M) | |||
|---|---|---|---|---|---|---|---|---|---|
| S. aureus | E. coli | C. albicans | MRSA | ||||||
| Brevinin-1GHa | 1.054 | 9.90 | +5 | 0.592 | 0.358 | 2 | 4 | 2 | 4 |
| Brevinin-1HSa | 1.262 | 9.85 | +4 | 0.728 | 0.377 | 3 | 24 | ND | ND |
| Brevinin-1JDc | 1.333 | 9.85 | +4 | 0.761 | 0.366 | 6 | 49 | 3 | 3 |
| Brevinin-1WY7 | 1.183 | 9.70 | +4 | 0.736 | 0.420 | ND | ND | ND | ND |
GRAVY, grand average of hydropathicity; pI, theoretical isoelectric point. GRAVY and pI were calculated by the online ProtParam tool (http:web.expasy.org/protparam) [25]. <H>, hydrophobicity; <μH>, hydrophobic moment; Net charge, <H> and <μH> were calculated by the online analysis tool, HeliQuest (http://heliquest.ipmc.cnrs.fr) [26]. ND, not detected. MICs came from publications [27,28].