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. 2018 Oct 15;201(10):3006–3016. doi: 10.4049/jimmunol.1701402

FIGURE 1.

FIGURE 1.

Requirement of IL-4 for the induction of Langerin+ cells from CD14+ monocytes in the presence of GM-CSF and TGF-β1. (A) Requirement of IL-4–mediated stimulation for Langerin+ cells. Monocytes were cultured with 100 ng/ml GM-CSF and 10 ng/ml IL-4 or GM-CSF and TGF-β1 with or without 5 μg/ml DLL1, 10 ng/ml IL-4, 20 ng/ml IL-6, 10 ng/ml IL-13, or 200 ng/ml IL-15 for 6 d. The expression of Langerin and DC-SIGN was assessed by FACS analysis. The percentage of Langerin+ cells from four independent experiments is shown in the lower panel (+SEM). (B) Effects of IL-4 depletion from the culture condition on the differentiation of CD14+ monocytes into Langerin+ cells. Monocytes were cultured with 100 ng/ml GM-CSF and 10 ng/ml TGF-β1 without 10 ng/ml IL-4 or completely washed out from culture medium at different times on 6 d. The expression of Langerin and DC-SIGN was assessed at day 6 by FACS analysis. The percentage of Langerin+DC-SIGN− cells/Langerin+ cells from five independent experiments is shown in the lower panel (+SEM). *p < 0.05, **p < 0.01, ****p < 0.0001, Student t test.