Temporal differences in response to altered tension. (A-F) Wing discs expressing en-Gal4 UAS-dcr2 tub-Gal80ts Jub:GFP (green) in controls (A,B), UAS-Sqh.EE (C,D) and UAS-Rok-RNAi (E,F) stained for E-cad (red) and Dcr2 (blue). Larvae were cultured at 18°C, shifted to the restrictive temperature for Gal80ts (29°C) for 24 h before dissection at ages corresponding to 84 h (A,C,E) or 120 h (B,D,F) AEL as indicated. (G-L) Wing discs expressing en-Gal4 UAS-GFP (green) UAS-dcr2 tub-Gal80ts
ex-lacZ in controls (G,H), UAS-Sqh.EE (I,J) and UAS-Rok-RNAi (K,L) stained for expression of ex-lacZ (red/white) and DNA (blue). Larvae were cultured at 18°C, shifted to the restrictive temperature for Gal80ts (29°C) for 24 h before dissection at ages corresponding to 84 h (G,I,K) or 120 h (H,J,L) AEL as indicated. (M,N) Histograms showing relative posterior to anterior (P/A) Jub:GFP (M) and ex-lacZ (N) intensities, normalized to E-cad or DNA, respectively, for the indicated genotypes at 84 h and 120 h AEL. Error bars indicate 95% confidence interval. n=12 for all time points. Statistical comparisons are shown relative to the control. ns, P>0.05; *P≤0.05; ****P≤0.0001. Scale bars: 20 µm.