Fig. 5.

Increasing cell density reduces cytoskeletal tension and Yki activity. (A,D,E,G,I,K) Wing discs with posterior compartment composed of faster-growing wild-type (+/+) cells and the anterior compartment composed of slower-growing Minute (Rps17)/+ cells, at 132 h AEL, which corresponds to ∼108 h AEL in the wild type. The anterior compartment is marked by staining of β-galactosidase (A,B,D,E,G,I; blue) or His:RFP (K; blue). Wing discs were also stained for E-cad (red/white) or DNA (red). (A) Wing disc stained for E-cad (red/white), panel on the right shows a cell area color map (blue to violet, 0 µm² to 12 µm²). (D) Staining for cleaved Dcp1 (green) reveals modest amounts of cell death, similar between the two compartments, except for some clusters of cell death near the A-P compartment boundary. (E) Wing disc showing Zip:GFP (green). Upper panels show a z slice through the disc. Junctional Zip:GFP decreased in the posterior compartment. (G,I,K) Wing discs showing Jub:GFP (G) or stained for Yki (I) or ex-lacZ (K). (B) Control Minute/+ wing disc stained for E-cad (red/white), panel on the right shows a cell area color map (blue to violet, 0 µm² to 12 µm²). (C) Quantitation of average cell areas throughout the wing pouch of anterior and posterior compartments in control (M/+ posterior compartment) and experimental (+/+ posterior compartment) wing discs. (F,H,J,L) Histograms showing anterior and posterior relative levels of junctional Zip:GFP (F), junctional Jub:GFP (H), nuclear Yki (J) or ex-lacZ (L), normalized to E-cad (F,H) or DNA (J,L) in Minute/+ vs +/+ wing discs. n=12 for all experiments. Error bars indicate 95% confidence interval. ****P≤0.0001. Scale bars: 20 µm.