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. 2018 Oct 22;145(20):dev167346. doi: 10.1242/dev.167346

Fig. 1.

Fig. 1.

smFISH analysis of Cer3 and Cer8 RNA transcripts in WT adult germline. (A) Schematic of the gonad in C. elegans hermaphrodite adult. (B) Total siRNAs and HRDE-1-co-IP siRNAs at Cer3. Each dot represents a sequenced siRNA. Sense and antisense siRNAs were plotted separately as indicated. 5′- and 3′-LTR siRNAs cannot be distinguished due to the identical DNA sequences of the two LTRs. (C) Design of Cer3/gag and Cer8 smFISH probe sets. (D) Cer3/gag and Cer8 smFISH signals at different stages of adult germ cells. Wild-type (N2) hermaphrodite animals (23°C) were used (also for panels E-G). (E) An example of a pair of adjacent Cer3/gag smFISH dots (white arrow) in a pachytene nucleus. (F) Percentage of Cer3/gag or Cer8 smFISH-positive nuclei observed at different developmental stages of adult germ cells. Five biological replicates. Mean±s.d. is plotted. (G-G‴) Two-color smFISH analysis of Cer3/gag and Cer8 at the pachytene stage. G′-G‴ show magnifications of the boxed areas in G. (H-H‴) Schematic of the gld-1(q485) tumorous germline phenotype (H) and Cer3/gag smFISH in different regions of the gld-1(q485) gonad (H′-H‴). (I-I″) Schematic of the glp-1(ar202) proximal proliferative zone (I) and Cer3/gag smFISH in different regions of glp-1(ar202) gonad (25°C) (I′,I″). See Fig. S4 for smFISH profiles of the entire gonads. Scale bars: 5 μm.