Skip to main content
. 2018 Oct 22;145(20):dev167346. doi: 10.1242/dev.167346

Fig. 5.

Fig. 5.

Cer3 RNA expression during embryogenesis. (A) Cer3/gag smFISH in 4-cell, 8-cell, ∼26-cell, 300+ cell, and pre-hatching stages of WT and different mutant embryos as indicated. Insets show enlargements of the boxed areas. (B) Simultaneous detection of pie-1 promoter-driven GFP::H2B (immunofluorescence with anti-GFP) and Cer3/gag RNA with smFISH in a pre-hatching embryo. This strain carries the Ppie-1::GFP::H2B, but otherwise is wild type. Right-hand panels show enlargements of the boxed area in the left-hand panel. (C) Quantification of Cer3/gag smFISH spots in ∼26-cell embryos. Scale bars: 5 μm. Ten biological replicates. Mean±s.d. is plotted. *P<0.05 (t-test).