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Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and DNA counter-staining with DAPI (blue). (A) 8-cell stage control embryo. (B,C) Cas9+gRNAs+GFP injected embryos showing abnormal nuclear morphology (arrows) at 6- and 8-cell stage, respectively.
