Fig. 1.

Optimization of the zebrafish primary neural cell culture. (A) Images of cell cultures derived from 24 hpf and 48 hpf-aged embryos. Motor neurons in both cultures exhibited outgrowth of long processes (arrows). (B) No difference in motor neuron survival rate was evident for cells incubated at 37°C or 28°C after 1 day (cultures from 24 hpf larvae). (C) Motor neurons in cultures derived from de-yolked embryos exhibited shorter neurites compared to those derived from whole embryos. Note however that by 2 div, almost 100% of cells from the de-yolked cultures were non-viable (not shown). Scale bar: 10 µm.