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. 2018 Sep 26;7(10):bio035386. doi: 10.1242/bio.035386

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — The expression level of NAGLU and CMTM8 genes and the ELISA assays for NAGLU enzyme and HS. (A) The mRNA level of NAGLU. (B) The mRNA level of CMTM8. (C) NAGLU enzyme amount. (D) HS storage amount. Experiments were conducted in four tissues from the 2.8-year-old founder and nine 180-day-old F1 pigs. NAGLU^+/−, F₁ pigs that carry one insertion site at the NAGLU locus (n=3); NAGLU^+/+, wild-type pigs (n=3); CMTM8 transgenic, F₁ pigs that harbor one insertion site at the CMTM8 locus (n=3); wild-type pigs (n=3). Data are presented as mean±s.e. except the founder boar. *P<0.05; **P<0.01. Phenotypic difference was not evaluated between the founder and the other individuals as only one founder animal was tested.