The TgHD minipig model shows behavioral abnormalities consistent with early neurodegeneration. (A) Behavioral testing with tests specially designed for minipigs revealed a deficit in performing the tunnel test (P=0.04) and indications of a deficit in walking (nonsignificant). (B) Representative images (clockwise from top) of the balance beam, hurdle, seesaw and tunnel tests. Mann–Whitney test and two-way ANOVA, *P<0.05. Box plots and whiskers indicate minimum to maximum values, with hinges representing the 25th and 75th percentiles and the median indicated by the centerline. Sample size [female (F)+male (M)] distribution: balance beam, WT 4F+2M, TgHD 4F+2M; hurdle, WT 3F+2M, TgHD 4F+1M; seesaw, WT 3F+2M, TgHD 3F+2M; tunnel, WT 3F+2M, TgHD 4F+1M; walking, WT 4F+2M, TgHD 4F+2M.