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. 2018 Oct 24;11(10):dmm035949. doi: 10.1242/dmm.035949

Fig. 2.

Fig. 2.

Tissue-specific changes in genome integrity in the TgHD minipig model. (A) mtDNA damage analysis revealed lower damage levels in the basal ganglia in TgHD than in WT (P=0.01), but no changes in PBMCs or the frontal cortex. (B) nDNA damage analysis demonstrated that nDNA integrity is apparently unaffected in the TgHD model. (C) Quantification of mtDNA copy number shows a significant decrease in the frontal cortex (P=0.04) and, in contrast, an increase in the basal ganglia (P=0.01) in the TgHD minipigs, relative to WT. No differences were seen in PBMCs. (D) mtDNA mutation frequency analysis indicates higher levels in the basal ganglia in TgHD (nonsignificant) than in WT, but normal levels in PBMCs and the frontal cortex. (E) The methylation mark 5-me(dC) was increased in the frontal cortex in TgHD compared with WT (P=0.047). No differences in 5-me(dC) levels were seen in PBMCs or the basal ganglia. Student's t-test, *P<0.05. Box plots and whiskers indicate minimum to maximum values, with hinges representing the 25th and 75th percentiles and the median indicated by the centerline. Sample sizes: (A) basal ganglia, WT n=4, TgHD n=5; PBMCs, WT n=17, TgHD n=14; frontal cortex, WT n=10, TgHD n=7; (B) PBMCs, WT n=17, TgHD n=14; frontal cortex, WT n=10, TgHD n=7; basal ganglia, WT n=4, TgHD n=5; (C) frontal cortex, WT n=10, TgHD n=5; basal ganglia, WT n=4, TgHD n=5; PBMCs, WT n=17, TgHD n=13; (D) basal ganglia, WT n=2, TgHD n=2; PBMCs, WT n=7, TgHD n=4; frontal cortex, WT n=10, TgHD n=6; (E) frontal cortex, WT n=10, TgHD n=7; PBMCs, WT n=10, TgHD n=6; basal ganglia, WT n=2, TgHD n=5.