Fig. 4.

Influence of chemical compound administration through injection on HDR efficiency. Injection mixes containing the NT 120 S repair template were complemented with chemical compounds that either inhibit specific components of the NHEJ pathway, including SCR7, NU7441 and KU0060648, or that were shown to stimulate the HDR pathway, including RS1 and L755507. Five independent experiments were carried out and average total HDR rates are shown, split into two categories: perfect repair % (plain bars) and erroneous repair % (dashed bars). Error bars represent the s.e.m. for five independent biological replicates each consisting of a pooled sample of 20 embryos. Repair rates depicted in this graph are listed in Table S4. Statistical tests performed: independent samples t-test for normal distributed groups and the non-parametric independent samples Mann–Whitney U-test for non-normal distributed groups.