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. 2018 Oct 18;11(10):dmm035352. doi: 10.1242/dmm.035352

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Determination of germline transmission of precise base-pair substitutions introduced by CRISPR/Cas9-mediated HDR. For two of the four target sites included in this study (the slc2a10 and smad6a zebrafish genes), embryos injected with injection mixes containing the NT 120 S repair template were grown until adulthood. For each target site, eight adult fish (labeled founder fish # 1, # 2, … # 8) were screened for the presence of the precise base-pair substitutions located closest to the Cas9 cut site in their germ cells. Therefore, DNA was extracted from collected eggs or sperm, and subjected to NGS analysis. HDR rates are shown, split into two categories: perfect repair % (plain bars) and erroneous repair % (dashed bars). Repair rates depicted in this graph are listed in Table S6.