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. 2018 Oct 18;11(10):dmm035469. doi: 10.1242/dmm.035469

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Generation of patient-specific KI lines in zebrafish. (A) Stepwise procedure followed to establish the KI lines described in this study with corresponding minimal timeline for each step. (B) Schematics showing the targeted genomic sequence for the introduction of the c.A193G substitution resulting in the p.V65M in the kcnj8 genomic sequence. The PAM sequence is highlighted in gray, the specific section of the sgRNA is highlighted in cyan and the modified codon is underlined. Red text indicates substituted nucleotide. Note that in this case the substitution is located on the PAM sequence. (C) Sequencing traces for wild-type and heterozygous kcnj8^+/V65M zebrafish. Asterisk denotes the substituted nucleotide in the heterozygous kcnj8^+/V65M sequencing trace.