Figure 2.

VX-970 delays resolution of RT-induced DNA double strand breaks in TNBC cells but not normal breast epithelial cells. MCF10A (A) and BT-549 (C) cells were pretreated with vehicle (DMSO) or VX-970 (80 nM) for one hour prior to exposure to 2 Gy and stained for ƔH2AX and 53 BP1 foci using immunofluorescence. (B, D) The percentage of cells with > 10 ƔH2AX and 53BP1 nuclear foci was quantified at 1 hour and 24 hours following 2 Gy. Bars represent the mean ± SEM.