Figure 6:

SntB regulates global levels of histone modifications. A) Levels of histone modifications were assessed by western blot from cultures grown for 72 hours in YES media. B) The relative intensity of the bands corresponding to histone H4 hyper acetylation (H4Ac) were calculated in Adobe PhotoshopTM, and standardized to the loading control, histone H3. Values were normalized to wild type. The same analysis was done for C (H3K4me3) and D (H3K9K14Ac). The deletion of sntB showed an increase in histone H3 acetylation, and a minimal increase in H3K4me3. Histone H3 is used as a loading control.