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Genotyping pups for disruptions in Rag2 gene. The targeted locus was amplified from pups sired by the implanted male and subjected to MseI digestion. Animal 2f contained at least one allele that is resistant to MseI digestion (white arrow). The PCR product from animal 2f was TOPO cloned and sequenced which revealed this animal carries a mutant allele with a 27bp deletion.
