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. 2018 Nov 1;6:e5892. doi: 10.7717/peerj.5892

Table 2. Effects of synthetic plant defense elicitors (Actigard® & cis-jasmone) & Azadirachtin® applications on wheat stem sawfly (WSS) & Bracon spp. adult individuals (15 sweeps/plot) & parasitism percentage (mean ± SE) in winter wheat fields at the three study locations of Montana.

Treatment WSS adult number Parasitoid adult number Parasitism %
10 DAT 20 DAT 30 DAT 10 DAT 20 DAT 30 DAT
Knees
Water-ES 7.50 ± 0.87 3.25 ± 1.31 0.5 0 ± 0.29 0.25 ± 0.25 3.25 ± 1.65 0.25 ± 0.25 24.05 ± 3.20
Water-ELS 5.50 ± 0.63 3.00 ± 0.91 0.00 ± 0.00 1.25 ± 0.41 2.75 ± 1.7 2.25 ± 0.95 14.83 ± 7.80
Water-LS 5.00 ± 1.22 1.75 ± 0.75 0.00 ± 0.00 0.25 ± 0.29 2.25 ± 0.48 1.25 ± 0.41 17.91 ± 9.00
Actigard-ES 2.25 ± 0.75 3.25 ± 0.25 0.25 ± 0.25 0.50 ± 0.25 4.50 ± 1.55 1.00 ± 0.41 13.48 ± 3.00
Actigard-ELS 1.15 ± 0.05 2.50 ± 0.87 0.00 ± 0.00 0.50 ± 0.00 4.00 ± 1.08 1.50 ± 0.65 9.32 ± 5.30
Actigard-LS 4.25 ± 1.03 1.75 ± 0.75 0.00 ± 0.00 0.00 ± 0.50 2.50 ± 1.5 1.50 ± 0.5 15.62 ± 3.10
cis-jasmone-ES 5.00 ± 1.68 2.00 ± 0.41 0.00 ± 0.00 0.25 ± 1.25 6.00 ± 2.35 0.00 ± 0.00 7.77 ± 1.90
cis-jasmone-ELS 3.75 ± 1.18 3.5 ± 1.19 0.00 ± 0.00 0.50 ± 0.25 4.75 ± 2.06 1.75 ± 0.63 14.72 ± 4.70
cis-jasmone-LS 2.75 ± 1.49 1.75 ± 0.85 0.50 ± 0.29 1.00 ± 0.75 4.25 ± 0.85 0.75 ± 0.48 13.21 ± 6.20
Azadirachtin-ES 4.25 ± 1.49 3.00 ± 0.41 0.25 ± 0.25 0.50 ± 0.25 4.00 ± 0.91 1.50 ± 0.5 19.84 ± 8.00
Azadirachtin-ELS 2.00 ± 0.40 3.25 ± 1.11 0.25 ± 0.25 1.25 ± 0.75 5.25 ± 1.31 1.50 ± 0.5 8.33 ± 8.30
Azadirachtin-LS 3.75 ± 1.93 2.75 ± 1.11 0.25 ± 0.25 0.25 ± 0.75 2.75 ± 1.11 0.75± 0.48 11.08 ± 4.00
Statistical output χ112=16.30; P = 0.13 χ112=7.38; P = 0.77 χ112=11.75; P = 0.38 χ112=16.64; P = 0.47 χ112=5.80; P = 0.89 χ112=13.38; P = 0.27 χ112=0.97; P = 0.49
Choteau
Water-ES 11.25 ± 1.89 19.00 ± 1.96 1.25 ± 0.63 2.00 ± 1.08 2.00 ± 0.41 0.25 ± 0.25 2.42 ± 1.02
Water-ELS 11.75 ± 2.06 19.75 ± 2.29 1.50 ± 0.87 2.25 ± 1.11 1.75 ± 1.11 2.75 ± 1.11 2.03 ± 1.43
Water-LS 12.25 ± 1.80 13.50 ± 1.19 1.00 ± 0.41 1.50 ± 0.75 2.50 ± 1.25 1.50 ± 0.76 1.56 ± 1.56
Actigard-ES 5.75 ± 1.38 21.50 ± 3.77 0.50 ± 0.50 1.75 ± 1.03 2.00 ± 0.82 2.00 ± 1.22 1.9 ± 0.68
Actigard-ELS 8.50 ± 1.44 15.25 ± 1.89 1.75 ± 0.85 0.50 ± 0.29 2.50 ± 1.19 0.75 ± 0.48 3.49 ± 1.35
Actigard-LS 10.50 ± 2.53 13.00 ± 3.00 1.75 ± 0.75 2.75 ± 1.18 2.75 ± 1.31 1.50 ± 1.19 2.9 ± 1.39
cis-jasmone-ES 11.00 ± 2.04 20.25 ± 3.47 2.25 ± 0.85 1.00 ± 0.71 1.50 ± 0.50 1.25 ± 0.75 2.27 ± 0.40
cis-jasmone-ELS 10.50 ± 2.10 17.75 ± 5.36 2.50 ± 1.19 1.25 ± 0.48 4.25 ± 0.85 0.75 ± 0.48 0.40 ± 0.40
cis-jasmone-LS 9.25 ± 2.21 18.25 ± 3.68 1.75 ± 0.63 1.50 ± 0.65 2.75 ± 0.95 0.75 ± 0.48 0.00 ± 0.00
Azadirachtin-ES 8.75 ± 3.47 20.00 ± 4.36 3.00 ± 0.71 1.75 ± 1.18 1.50 ± 0.65 1.50 ± 0.65 0.00 ± 0.00
Azadirachtin-ELS 6.25 ± 0.82 15.75 ± 3.82 0.50 ± 0.50 2.75 ± 1.6 3.50 ± 0.50 1.00 ± 0.58 0.78 ± 0.78
Azadirachtin-LS 6.75 ± 1.93 19.00 ± 3.16 1.00 ± 0.00 1.00 ± 0.71 1.25 ± 0.75 1.50 ± 0.96 0.00 ± 0.00
Statistical output χ112=14.67; P = 0.20 χ112=9.33; P = 0.60 χ112=12.74; P = 0.31 χ112=5.85; P = 0.89 χ112=12.32; P = 0.34 χ112=7.24; P = 0.78 χ112=1.23; P = 0.30
Conrad
Water-ES 3.00 ± 0.70 1.50 ± 0.95 0.50 ± 0.29 0.25 ± 0.25 0.25 ± 0.25 0.25 ± 0.25 10.54 ± 2.63
Water-ELS 2.50 ± 0.65 2.00 ± 1.08 0.00 ± 0.00 1.00 ± 0.41 0.75 ± 0.48 0.75 ± 0.48 7.39 ± 2.50
Water-LS 1.25 ± 0.48 1.00 ± 0.70 0.75 ± 0.48 1.00 ± 0.29 1.50 ± 0.29 0.00 ± 0.00 15.56 ± 3.04
Actigard-ES 2.67 ± 1.15 1.00 ± 0.70 0.25 ± 0.25 0.25 ±0.25 1.00 ± 0.71 0.25 ± 0.25 21.94 ± 3.94
Actigard-ELS 0.75 ± 0.48 0.75 ± 0.48 0.25 ± 0.25 0.00 ± 0.00 1.50 ± 0.87 0.5 ± 0.29 19.31± 6.97
Actigard-LS 1.75 ± 1.03 2.25 ± 1.31 0.00 ± 0.00 0.50 ±0.5 0.25 ± 0.25 0.00 ± 0.00 16.12 ± 1.98
cis-jasmone-ES 1.25 ± 0.63 1.00 ± 0.70 0.75 ± 0.48 1.25 ± 1.25 1.75 ± 0.85 0.0 0 ± 0.00 8.89 ± 5.11
cis-jasmone-ELS 2.50 ± 0.87 1.25 ± 0.75 0.25 ± 0.25 0.25 ± 0.25 1.25 ± 0.63 0.75 ± 0.25 10.92 ± 5.19
cis-jasmone-LS 2.50 ± 0.64 0.25 ± 0.25 0.00 ± 0.00 1.25 ± 0.75 1.50 ± 0.87 0.25 ± 0.25 18.08 ± 3.71
Azadirachtin-ES 1.00 ± 0.71 1.25 ± 0.75 0.25 ± 0.25 0.25 ± 0.25 1.00 ± 0.71 0.75 ± 0.75 7.67 ± 3.35
Azadirachtin-ELS 1.50 ± 0.87 0.75 ± 0.48 0.00 ± 0.00 1.25 ± 0.75 0.25 ± 0.25 0.25 ± 0.25 11.77 ± 6.06
Azadirachtin-LS 1.75 ± 0.85 0.75 ± 0.48 0.00 ± 0.00 0.75 ± 0.75 0.25 ± 0.25 0.25 ± 0.25 12.38 ± 6.79
Statistical output χ112=10.42; P = 0.49 χ112=3.66; P = 0.98 χ112=10.41; P = 0.51 χ112=13.70; P = 0.25 χ112=8.33; P = 0.68 χ112=16.3010.65; P = 0.47 χ112=16.301.05; P = 0.43

Notes:

The number of replicates per treatment was four.

DAT, Days after the initial treatment application (i.e., chemical sprayed at wheat stem sawfly egg stage); ES, Egg stage; ELS, Egg and larval stages; LS, Larval stage.