Fig. 6.
The role of NetPhorest in phosphoproteomics and modeling of phosphorylation-dependent signaling networks. The NetPhorest atlas of consensus linear motifs can be used for designing synthetic peptides for the development of kinase- or family-specific antibodies (for example, pS/T-Q), will replace Scansite (13) and NetPhosK (20) as the motif component of the NetworKIN resource (http://networkin.info) (12, 57), and can be used to detect biases arising from the enrichment procedures commonly used in phosphoproteomics [for example, phosphoramidate chemistry (PAC), immobilized metal affinity chromatography (IMAC), and titanium oxide (TiO2) (58)]. The NetPhorest Web site (http://netphorest.info) provides the means to classify phosphorylation sites on the basis of consensus sequence motifs.
