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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Exp Eye Res. 2018 Jun 28;176:121–129. doi: 10.1016/j.exer.2018.06.028

Fig. 6.

Fig. 6

Immunofluorescence images of typical TUNEL+ regions thresholded by manual and automated MCT methods (A). Immunofluorescence images of hotspot TUNEL+ regions thresholded by manual and automated MCT methods (B). For both A and B, the first column shows the cropped region with no analysis outlines (top) and the outlines of the manual MCT method (bottom). On subsequent columns the top row is thresholded with the MaxEntropy algorithm while the bottom row is thresholded with the RenyiEntropy algorithm. The second column uses the IsoData algorithm to threshold the DAPI+ cells, the third column uses the Li algorithm, the fourth column uses the Mean algorithm, and the fifth column uses the Otsu algorithm. Scale bar denotes 50μm.