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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Exp Eye Res. 2018 Jun 28;176:121–129. doi: 10.1016/j.exer.2018.06.028

Fig. 7.

Fig. 7

TUNEL+ cell counts for all analysis methods. Images 1 through 10 represent hotspot regions while images 11 through 20 represent typical regions. Hotspot cell count scale to the left y-axis from 0 to 600 while typical cell counts scale to the right y-axis from 0 to 300. The primary analysis methods are represented by dots and full lines (RA Toolkit standard – open red circle, RA Toolkit high – filled red circle, ImagePro – open black circle, MCT: Manual – filled black circle). All methods utilizing the MaxEntropy algorithm are represented by triangles while methods utilizing the RenyiEntropy algorithm are represented by squares. The count using MaxEntropy without the MCT method is represented by a black triangle with a dotted line while the count using RenyiEntropy without the MCT method is represented by a black square with a dashed line. The subsequent automated MCT methods are colored according to the DAPI thresholding algorithm used (IsoData – Red, Li – Green, Mean – Purple, Otsu – Blue).