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All methods used to analyze selected 20 hotspot and typical TUNEL images.
| Primary Analysis
| |||||
|---|---|---|---|---|---|
| RA Toolkit: Standard (1) & High (2) | ImagePro Method (3) | MCT Method: Manual (4) | |||
|
| |||||
| MCT Automated Analysis | DAPI Thresholding Algorithm
|
||||
| IsoData (ID) | Li | Mean | Otsu | ||
| TUNEL Thresholding Algorithm | MaxEntropy (ME) | ME+ID (5) | ME+Li (6) | ME+Mean (7) | ME+Otsu (8) |
| RenyiEntropy (RE) | RE+ID (9) | RE+Li (10) | RE+Mean (11) | RE+Otsu (12) | |
Abbreviations: ME – MaxEntropy, RE – RenyiEntropy, ID – IsoData.
