A. In response to various stress stimuli, Msn2 proteins become dephosphorylated and translocate into the nucleus to activate downstream gene expression. PP1: Protein phosphatase 1, PKA: cAMP-dependent protein kinase A, STRE: stress response element. B. Msn2 nuclear localization trajectory of a cell, showing how amplitude, frequency, and duration of Msn2 nuclear localization are quantified. The dashed horizontal line denotes the threshold level above which there would be an Msn2 nuclear localization event. ni denotes the number of above-the-threshold localization events. T denotes the length of time interval used for the calculation of frequency. Figure panel was taken from 16.