Table 4.
Agreement in identification of common genus between DNA sequencing and culture method in 22 dust samples
| Genus* | Kappa statistics** |
Total detection % (no. of samples) |
% of common detection |
% of exclusive detection |
||
|---|---|---|---|---|---|---|
| DNA | Culture | DNA | Culture | |||
| Alternaria | −0.08 | 27.3 (6) | 4.6 (1) | 0 | 27.3 | 4.6 |
| Aspergillus | 0.27 | 50 (11) | 77.3 (17) | 45.5 | 4.6 | 31.8 |
| Aureobasidium | 0 | 72.7 (16) | 50 (11) | 36.4 | 36.4 | 13.6 |
| Botrytis | −0.08 | 18.2 (4) | 4.6 (1) | 0 | 18.2 | 4.6 |
| Chaetomium | −0.08 | 4.6 (1) | 27.3 (6) | 0 | 4.6 | 27.3 |
| Cladosporium | 0.07 | 18.2 (4) | 86.4 (19) | 18.2 | 0 | 68.2 |
| Epicoccum | 0.14 | 54.6 (12) | 77.3 (17) | 45.5 | 9.1 | 31.8 |
| Fusarium | −0.12 | 13.6 (3) | 9.1 (2) | 0 | 13.6 | 9.1 |
| Mucor | −0.09 | 40.9 (9) | 27.3 (6) | 9.1 | 31.8 | 18.2 |
| Penicillium§ | – | 36.4 (8) | 100 (22) | 36.4 | 0 | 63.6 |
| Phoma | −0.06 | 22.7 (5) | 68.2 (15) | 13.6 | 9.1 | 54.6 |
| Pithomyces | 0.11 | 72.7 (16) | 13.6 (3) | 13.6 | 59.1 | 0 |
*
Genus which was detected in more than 20% of samples by either culture or DNA sequencing method was only included in this analysis.
**
Kappa score: no agreement (<0); slight agreement (0.01–0.20); fair agreement (0.21–0.40); moderate agreement (0.41–0.60); substantial agreement (0.61–0.80); almost perfect agreement (0.81–0.99)1.
§
All samples were positive from culture method. Therefore, Kappa score could not be calculated.