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. 2018 Oct 29;6:483. doi: 10.3389/fchem.2018.00483

Table 10.

Sterols and phytols in Wolffia species.

Clone Total sterols Phytol Dihydro-phytol Campesterol Stigmasterol β-Sitosterol Sitostanol Δ5-Avena-sterol Δ7-Sitosterol 24-Methylene cycloartanol
W. angusta 8878 2.8 3.4 0.2 11 12 75 n.d. 2 n.d. n.d.
W. arrhiza 8618 5.2 1.8 n.d. 9 n.d. 84 7 n.d. n.d. n.d.
W. arrhiza 8853 4.3 3.9 0.7 8 5 73 14a n.q. n.d. n.d.
W. arrhiza 9528 4.6 4.8 0.5 11 2 77 10a n.q. n.d. n.d.
W. australiana 7540* 3.8 4.9 0.5 7 20 61 n.d. 6 n.d. 2
W. borealis 9123 3.4 4.5 1.3 8 4 81 n.d. 3 n.d. 4
W. brasiliensis 7925 3.7 5.2 n.d. 7 17 73 n.d. 3 n.d. n.d.
W. columbiana 7155* 4.4 4.7 n.d. 5 1 73 n.d. n.q.b 17 n.d.
W. cylindracea 9056* 4.6 3.6 0.4 8 1 77 11a n.q. n.d. 2
W. elongata 9188* 5.3 3.8 0.7 13 17 57 n.d. n.q.b n.d. 7
W. globosa 5514 3.8 4.8 0.5 7 4 79 n.d. 3 2 5
W. globosa 5515 3.2 2.5 0.6 6 5 78 n.d. 2 3 6
W. globosa 5537 3.8 3.3 1.1 5 3 82 n.d. 3 2 5
W. globosa 9498 2.4 2.3 1.1 12 5 77 n.d. 6 n.d. n.d.
W. microscopica 2005* 3.6 3.9 0.5 12 15 60 n.d. 9 n.d. 3
W. neglecta 9149* 3.9 3.0 0.5 7 4 80 n.d. 4 n.d. n.d.
RSD (%) 7 6 4 4 4 1 3 5 3 3
Average 4.1 3.9 0.9 8 7 70 9 4 5 4
± SD 1.1 1.1 0.9 3 6 19 4 2 6 2

Total sterols, phytol and dihydrophytol are given in g/100 g fat. LOD = 0.007 g/100 g fat, LOQ = 0.02 g/100 g fat. Sterol components were given as % of total sterols. n.d., not detectable; n.q., not quantifiable. Limit of detection (LOD) and Limit of quantification (LOQ) were calculated as three and ten times the noise signal of a blank. For further explanations see Table 2.

a

Coelution with Δ5-avenasterol,

b

Coelution with β-amyrin (~4–5 mg/g fat)

*

further minor sterols contributing up to 6% to the total sterol content which are not listed here were 24- methylene cholesterol, cycloartenol, Δ7-stigmastenol and Δ5,24(25)-stigmastadienol

Method precision < 10%, calculated for each substance as relative standard deviation (RSD) within the actual measurement concentration (nsample = 2; 2 ≤ nbatch ≤ 16) RSDbatch[%]= RSDsample¯= 1nbatchi=1nbatchSDsamplex¯ ·100