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. 2018 Oct 29;9:2502. doi: 10.3389/fimmu.2018.02502

Figure 1.

Figure 1

Low frequency of circulating non-activated CD161-expressing CD8+ T-cells in PLWH. (A) Gating strategy for the detection of circulating CD161-expressing CD4+ and CD8+ T-cells. Representative pseudocolor plots from a healthy individual and a subject living with HIV are shown. Peripheral blood lymphocytes were first gated and duplicates were excluded (FSC-A/FSC-H). T-cells were identified as CD3+. The numbers next to the gates indicate the percentage of the respective population. At the right of each plot is shown the summary of the frequency of CD161-expressing CD4+ and CD8+ T-cells in healthy individuals and PLWH. (B) Cell ACCENSE t-SNE plots of CD3, CD4, CD8, CD161, HLA-DR, and CD38 expression in cells from a representative healthy individual and a subject living with HIV (M = 30,000). The black and red circles indicate, respectively, CD161-positive CD4+ and CD8+ T-cells. (C,D) Frequency of HLA-DR- and/or CD38-expressing CD161+ (C) and CD161hi (D) CD8+ T-cells in healthy individuals and PLWH. In (A,C,D) the P-value of the Mann-Whitney test is shown. NS, Not statistically significant.