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. 2018 Oct 29;9:2512. doi: 10.3389/fimmu.2018.02512

Figure 5.

Figure 5

GATA3-AS1 RNA forms an R-Loop with the central intron of GATA3-AS1. (A) Predictive R-Loop formations according to the R-Loop database. The largest and most likely R-Loop is circled, located between regions 2 and 3 of GATA3-AS1. (B) DRIP assay completed with RNA isolation. Results are expressed as fold enrichment relative to the RNase H negative control. Chromatin was treated with RNase to remove R-Loops as an additional control, mean ± S.D. (n = 3). *P < 0.05. (C) DRIP assay similar to (B), but DNA was isolated from the R-Loop immunoprecipitates. PRO1 and 2 represent the shared promoter region, INT is the central intron of GATA3-AS1, and Post3' is past the GATA3-AS1 transcriptional end site. Statistical evaluations were performed using Students t-test vs. Rnase H negative control. All data represent mean± S.D. as fraction of input (n = 4). *P < 0.05.