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. 2018 Oct 29;9:2556. doi: 10.3389/fmicb.2018.02556

FIGURE 4.

FIGURE 4

Effects of temperature, pH and glucose concentration on the activity of the β-glucosidase Lfa2. (A) Effect of temperature on the activity of Lfa2 was determined in pH 5.5 (citric acid-phosphate buffer 50 mM) in a range of temperature from 25 to 70°C. (B) Effect of pH on the activity of Lfa2 was determined in the range of pH 2.0–8.0 at 50°C using citric acid-phosphate buffer (50 mM). (C) Influence of increasing glucose concentrations (0–1.35 M) on Lfa2 activity was performed in citric acid-phosphate buffer 50 mM pH 5.5 at 50°C. All enzymatic properties were determined using pNPβG as substrate. All measurements were done in triplicates. Error bars show SD. Activity at 100% refers to a specific activity of 9.6 μmol min-1 mg-1.