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. 2018 Oct 29;9:2556. doi: 10.3389/fmicb.2018.02556

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Copyright © 2018 Alves, Meleiro, Silva, Westmann and Guazzaroni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative activity of Lfa2 in different concentrations of lignocellulose pretreatment-derived compounds. Different concentrations of furfural (0–1%), 5-hydroxymethyl furfural (5-HMF) (0–1%), vanillin (0–2%), and 4-hydroxybenzoic acid (4-HBA) (0–2%) on Lfa2 activity were analyzed using 4 mM pNPβGlu as substrate in citric acid-phosphate buffer 50 mM pH 5.5 at 50°C. Control experiments were performed in absence of lignocellulose pretreatment-derived compounds. All measurements were done in triplicates. Error bars show SD. Activity at 100% refers to a specific activity of 7.9 μmol min^-1 mg^-1.