Table 1.
Description of the ORFs contained in pLFA2 plasmid and their sequence similarities.
| Plasmid name [insert (bp)] | % G + C | ORFa | Length (aa)b | Closest similar protein (length in aa) | Organism | E-value | Identity (%) | Putative function | No. Of TMHc | Signal Peptide |
|---|---|---|---|---|---|---|---|---|---|---|
| pLFA2 (5,868) | 53,2 | 1 | 577 | hypothetical protein A2W03_04630 [OGD23027.1] (641) | Candidatus Aminicenantes bacterium RBG_16_63_16 | <5.00E-133 | 56 | Acetyl xylan esterase | 0 | No |
| 2 | 785 | Glycosyl hydrolase family 3 [WP_041974888.1] (787) | Pyrinomonas methylaliphatogenes | <5.00E-133 | 59 | Glycosyl hydrolase family 3 | 0 | Yesd | ||
| 3∗ | 385∗∗ | Carboxypeptidase [OGU07865.1] (461) | Gemmatimonadetes bacterium RBG 16668 | 5.00E-133 | 62 | Carboxypeptidase | 1 | No | ||
aOpen reading frame. bAmino acids. cNumber of transmembrane helices (TMH) predicted by using the TMHMM program (http://www.cbs.dtu.dk/services/TMHMM-2.0/). dAccording to SignalP software (http://www.cbs.dtu.dk/services/SignalP/), the clivage site is between aminoacids 22 and 23; considering an analysis for Gram-negative bacteria. ∗Asterisks indicate incomplete ORFs and ∗∗proteins truncated at C-terminal end.