Table 2.

Influence of metal ions, chelating agent, organic solvents and salt on Lfa2 activity.

Compound	Relative activity (%)
Control (no addictives)	100 ± 6.1
10 mM MgCl2	130.8 ± 4.3
10 mM CoCl2	134.1 ± 11.2
10 mM MnSO4	109.4 ± 0.3*
10 mM CuSO4	5.8 ± 1.3
10 mM CaCl2	105.7 ± 5.5*
10 mM NiSO4	92.1 ± 3*
10 mM ZnSO4	94.6 ± 8.8*
10 mM FeSO4	17.4 ± 4.1
10 mM EDTA	79.3 ± 1.6
250 mM NaCl	137.4 ± 6.5
1 M NaCl	135.9 ± 6.1
10% DMSO	86.6 ± 3.1*
25% DMSO	65.5 ± 3
50% DMSO	21.3 ± 1.2
10% EtOH	170.1 ± 1.7
25% EtOH	59.1 ± 2.4
50% EtOH	35.6 ± 0.8

^∗No significant difference when compared with the control (no addictives) as revealed by Tukey test, p < 0.05. Activity at 100% refers to a specific activity of 7.9 μmol min^-1 mg^-1 using pNPβGlu as substrate.