Table 4.
Kinetic parameters of the purified β-glucosidase Lfa2.
| Substrate | Vmáx (μmol min-1 mg-1) | KM (mM) | kcat (s-1) | kcat/KM (s-1 M-1) |
|---|---|---|---|---|
| pNPβGlu | 9.4 ± 0.2 | 0.76 ± 0.06 | 13.2 ± 0.2 | 17.4 × 103 |
| Cellobiose | 16.2 ± 1.2 | 75.3 ± 11.6 | 22.8 ± 1.7 | 3.02 × 102 |
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