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. 2018 Oct 29;8:476. doi: 10.3389/fonc.2018.00476

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Copyright © 2018 Pedra, Galdino, da Silva, Ramos, Bona, Soares, Azambuja, Canuto, de Brito, Ribeiro, Souza, Cunico, Stefanello, Spanevello and Braganhol.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of crude extracts, fractions and Sch-642305 effect on C6 glioma cell death. C6 glioma was exposed to eDCM (1 μg/mL), eEtAc (50 μg/mL), F3_EtAc (25 μg/mL) and Sch-642305 (1 μg/mL). Cells were treated for 48 h and subsequently analyzed by flow cytometry using annexin V/PI double staining to determine cell death by apoptosis, late apoptosis or necrosis. (A–E). The percentage of viable cells is represented by the lower left quadrant; the percentage of apoptosis is represented by the lower right, the percentage of late apoptosis is represented by the upper right and the percentage of necrosis is represented by the upper left; (F) representative graph of cell death analysis. ^{a, b, d}Significantly different from viable, apoptotic, and late apoptotic of control cells, respectively (P < 0.001).