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. 2018 Oct 29;8:476. doi: 10.3389/fonc.2018.00476

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Copyright © 2018 Pedra, Galdino, da Silva, Ramos, Bona, Soares, Azambuja, Canuto, de Brito, Ribeiro, Souza, Cunico, Stefanello, Spanevello and Braganhol.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of crude extracts, fractions and Sch-642305 effect on C6 glioma cell cycle. C6 glioma cells exposed to eDCM (1 μg/mL), eEtAc (50 μg/mL), F3_EtAc (25 μg/mL) and Sch-642305 (1 μg/mL). Cells were treated for 48 h and subsequently analyzed by flow cytometry using PI staining to determine the distribution of cells on cell cycle. The percentage of cells on sub-G1, G1, S, and G2/M phases were shown in the graph.^{a, b, c, d} Significantly different from Sub-G1, G1, S, and G2/M phases of control cells, respectively (P < 0.001).