Table 1.

List or primers used for the detection of T3SS and T6SS effectors.

Secretion system	Gene	Primers
		Name	Sequences
T3SS	pscC∗	F pscC	5′-ccaggcctgccttacgactat-3′
		R pscC	5′-tcgtagtaccagcccgaggtt-3′
	exoS	R exoS	5′-atgccggtgtagagaccaag-3′
		F exoS	5′-aggtcagcagagtatcggc-3′
	exoT	R exoT	5′-tcgatcgatatctcgctgac-3′
		F exoT	5′-tgaacaggtcgtgaagacc-3′
	exoU	R exoU	5′-acgtaacagagctacgttgg-3′
		F exoU	5′-ttgatcatcagcagttactcg-3′
	exoY	R exoY	5′-tgccatagaatccgtcctcg-3′
		F exoY	5′-atgacctcttcctggtagcg-3′
H1-T6SS	tssB1∗	F tssB1	5′-gtgcagatcgagtacgacg-3′
		R tssB1	5′-tcttgccgtccatgtaggt-3′
	tse1	R tse1	5′-agggagtagacgtagtag-3′
		F tse1	5′-aagtacccgatgtgctggtg-3′
	tse2	R tse2	5′-acgaagttgtccagttgg-3′
		F tse2	5′-ttaaggtgaagcaggacagc-3′
	tse3	R tse3	5′-ttgctgtacttggtgacgg-3′
		F tse3	5′-atccagggtccgttcag-3′
H2-T6SS	tssB2∗	F tssB2	5′-atggccaaagaaggctcggt-3′
		R tssB2	5′-tggtgcttggccaggacctc-3′
	pldA	R pldA	5′-tgtcgatcaccacggatttc-3′
		F pldA	5′-tatgacttcgaaaccatgctcg-3′
H3-T6SS	tssB3∗	F tssB3	5′-gtacgcagcacaagctggac-3′
		R tssB3	5′-ttgaccgggtcgaagtcctc-3′
	pldB	R pldb	5′-ttcgatgttgttttcctggcc-3′
		F pldb	5′-aagaccacctacgacatgatg-3′

^∗genes encoded a structural protein of the secretion system (T3SS or T6SS).