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. 2018 Oct 29;9:1522. doi: 10.3389/fpls.2018.01522

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Copyright © 2018 Khedia, Agarwal and Agarwal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In silico analysis of AlNAC4 sequence. (A) Schematic representation of AlNAC4 protein with A-E sub-domains, (B) the relationship among NAC proteins is represented using N-J phylogenetic tree constructed by DNAMAN software. Scale represents the branch length. The sequences used from OsNAC4 (AB028183.1), MlNAC4 (KM017003.1), TaNAC4 (GQ985329), ATAF1 (CAA52771.1), OsNAC5 (AB028184.1), CUC2 (BK007973.1), TaNAC7 (HM027572), GRAB2 (AJ010830), OsNAC7 (BAA89801.1), NTL4 (NM_111885), NTL11 (NM_120523), OsNTL5 (NM_001069053), and OsNTL6 (NM_001055094). Conserved motifs were identified by MEME software (multilevel consensus sequences for the MEME defined motifs are listed in Table 1), (C) diagrammatic representation of the position of introns (203–352; 637–734bp) and exons of AlNAC4. The scale underneath the diagram represents 100 bp.