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. 2018 Oct 29;9:1522. doi: 10.3389/fpls.2018.01522

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Copyright © 2018 Khedia, Agarwal and Agarwal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Schematic representation of the AlNAC4 ORF cloned in pGBKT7 vector, (B) transactivation assay for AlNAC4 gene, (i) Transformed yeast cell (AH109) containing pGBKT7 + AlNAC4 and pGBKT7 alone grown on SD/-Trp medium, (ii) Transformed yeast cell (AH109) containing pGBKT7 + AlNAC4 showing growth on SD/-Trp/-His/-Ura medium, while only pGBKT7 did not grow, (iii) Yeast cells transferred on filter paper showed β-galactosidase activity using X-gal staining. Electro Mobility Shift Assay of AlNAC4 protein with heterologous, NACBST (tandem) and NACBS probes showing specific binding with NACBST and NACBS (C). The detailed kinetics of AlNAC4 protein with NACBST (tandem, D) and NACBS (E) probes.